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Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule

Xiao, C., Tuthill, T. J., Kelly, C. M. B., Challinor, L. J., Chipman, P. R., Killington, R. A., Rowlands, D. J., Craig, Alister ORCID: and Rossmann, M. G. (2004) 'Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule'. Journal of Virology, Vol 78, Issue 18, pp. 10034-10044.

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Intercellular adhesion molecule 1 (ICAM-1) is the cellular receptor for the major group of human rhinovirus serotypes, including human rhinovirus 14 (HRV14) and HRV16. A naturally occurring variant of ICAM-1, ICAM-1(Kilifi), has altered binding characteristics with respect to different HRV serotypes. HRV14 binds to ICAM-1 only transiently at physiological temperatures but forms a stable complex with ICAM-1(Kilifi). Conversely, HRV16 forms a stable complex with ICAM-1 but does not bind to ICAM-1(Kilifi). The three-dimensional structures of HRV14 and HRV16, complexed with ICAM-1, and the structure of HRV14, complexed with ICAM-1(Kilifi), have been determined by cryoelectron microscopy (cryoEM) image reconstruction to a resolution of approximately 10 Angstrom. Structures determined by X-ray crystallography of both viruses and of ICAM-1 were fitted into the cryoEM density maps. The interfaces between the viruses and receptors contain extensive ionic networks. However, the interactions between the viruses and ICAM-1(Kilifi) contain one less salt bridge than between the viruses and ICAM-1. As HRV16 has fewer overall interactions with ICAM-1 than HRV14, the absence of this charge interaction has a greater impact on the binding of ICAM-1(Kilifi) to HRV16 than to HRV14.

Item Type: Article
Uncontrolled Keywords: intercellular-adhesion molecule-1 common cold virus cellular receptor crystal-structure 3-dimensional reconstruction angstrom resolution functional-analysis structural-analysis coxsackievirus b3 antiviral agents
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 268.5 Antiviral agents (General)
QW Microbiology and Immunology > Viruses > QW 160 Viruses (General). Virology
WC Communicable Diseases > Virus Diseases > Viral Respiratory Tract Infections. Respirovirus Infections > WC 510 Common cold
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):
Depositing User: Sarah Lewis-Newton
Date Deposited: 02 May 2013 15:23
Last Modified: 17 Jul 2019 14:14


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