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Fraser, Alice, Greenland-Bews, Caitlin, Kelly, Daniel, Williams, Christopher, Body, Richard, Adams, Emily 
ORCID: https://orcid.org/0000-0002-0816-2835, CubasAtienzar, Ana, Edwards, Thomas and Allen, David J.
(2023)
'A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations'. Scientific Reports, Vol 13, Issue 1, e3887.
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41598_2023_Article_30754.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities.
| Item Type: | Article |
|---|---|
| Corporate Authors: | LSTM Diagnostics Group |
| Subjects: | WF Respiratory System > WF 140 Diseases of the respiratory system (General) |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1038/s41598-023-30754-1 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 17 Mar 2023 16:06 |
| Last Modified: | 30 Jun 2023 12:26 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/22103 |
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