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A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations

Fraser, Alice, Greenland-Bews, Caitlin, Kelly, Daniel, Williams, Christopher, Body, Richard, Adams, Emily ORCID:, CubasAtienzar, Ana, Edwards, Thomas and Allen, David J. (2023) 'A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations'. Scientific Reports, Vol 13, Issue 1, e3887.

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The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities.

Item Type: Article
Corporate Authors: LSTM Diagnostics Group
Subjects: WF Respiratory System > WF 140 Diseases of the respiratory system (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):
SWORD Depositor: JISC Pubrouter
Depositing User: JISC Pubrouter
Date Deposited: 17 Mar 2023 16:06
Last Modified: 30 Jun 2023 12:26


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