Molecular and serological evidence of Crimean-Congo hemorrhagic fever orthonairovirus prevalence in livestock and ticks in Cameroon

Tchetgna, Huguette Simo, Yousseu, Francine S, Cosset, François-Loïc, Bezerra de Freitas, Natalia, Kamgang, Basile, McCall, Philip ORCID: https://orcid.org/0000-0002-0007-3985, Ndip Ndip, Roland, Legros, Vincent and Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673 (2023) 'Molecular and serological evidence of Crimean-Congo hemorrhagic fever orthonairovirus prevalence in livestock and ticks in Cameroon'. Frontiers in Cellular and Infection Microbiology, Vol 13, e1132495.

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Abstract

Introduction: Despite a high fatality rate in humans, little is known about the occurrence of Crimean-Congo hemorrhagic fever virus (CCHFV) in Cameroon. Hence, this pioneer study was started with the aim of determining the prevalence of CCHFV in domestic ruminants and its potential vector ticks in Cameroon.

Methods: A cross-sectional study was carried out in two livestock markets of Yaoundé to collect blood and ticks from cattle, sheep, and goats. CCHFV-specific antibodies were detected in the plasma using a commercial ELISA assay and confirmed using a modified seroneutralization test. Ticks were screened for the presence of orthonairoviruses by amplification of a fragment of the L segment using RT-PCR. Phylogeny was used to infer the genetic evolution of the virus.

Results: Overall, 756 plasma samples were collected from 441 cattle, 168 goats, and 147 sheep. The seroprevalence of CCHFV was 61.77% for all animals, with the highest rate found in cattle (433/441, 98.18%) followed by sheep (23/147, 15.65%), and goats (11/168, 6.55%), (p-value < 0.0001). The highest seroprevalence rate was found in cattle from the Far North region (100%). Overall, 1500 ticks of the Rhipicephalus (773/1500, 51.53%), Amblyomma (341/1500, 22.73%), and Hyalomma (386/1500, 25.73%) genera were screened. CCHFV was identified in one Hyalomma truncatum pool collected from cattle. Phylogenetic analysis of the L segment classified this CCHFV strain within the African genotype III.

Conclusion: These seroprevalence results call for additional epidemiological studies on CCHFV, especially among at-risk human and animal populations in high-risk areas of the country.

Item Type:	Article
Subjects:	QX Parasitology > QX 20 Research (General) QX Parasitology > QX 45 Host-parasite relations QX Parasitology > Arthropods > QX 479 Ticks WC Communicable Diseases > WC 20 Research (General) WC Communicable Diseases > Virus Diseases > Viral Hemorrhagic Fevers. Other Virus Diseases > WC 534 Viral hemorrhagic fevers
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.3389/fcimb.2023.1132495
Depositing User:	Maria Grimes
Date Deposited:	06 Apr 2023 14:36
Last Modified:	11 Apr 2023 09:02
URI:	https://archive.lstmed.ac.uk/id/eprint/22252

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