Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

von Blumroder, D., Schares, G., Norton, R., Williams, Diana J.L., Esteban-Redondo, I., Wright, S., Bjorkman, C., Frossling, J., Risco-Castillo, V., Fernandez-Garcia, A., Ortega-Mora, L. M., Sager, H., Hemphill, A., van Maanen, C., Wouda, W. and Conraths, F. J. (2004) 'Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines'. Veterinary Parasitology, Vol 120, Issue 1-2, pp. 11-22.

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Abstract

Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	neospora caninum enzyme-linked immunosorbent assay indirect fluorescent antibody test cut-off tg-roc test standardisation linked immunosorbent assays dairy-herd elisa antibodies abortion cattle cows outbreak epidemic sera
Subjects:	QW Microbiology and Immunology > Reference Works. General Immunology > QW 520 Research (General) QX Parasitology > QX 20 Research (General)
Faculty: Department:	Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008)
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.vetpar.2003.12.010
Depositing User:	Martin Chapman
Date Deposited:	29 Aug 2012 13:34
Last Modified:	06 Feb 2018 13:03
URI:	https://archive.lstmed.ac.uk/id/eprint/2257

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