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Acute rotavirus infection is associated with the induction of circulating memory CD4 + T cell subsets

Malamba-Banda, Chikondi, Mhango, Chimwemwe, Benedicto-Matambo, Prisca, Mandolo, Jonathan, Chinyama, End, Kumwenda, Orpha, Barnes, Kayla G., Cunliffe, Nigel A., Iturriza-Gomara, Miren, Jambo, Kondwani ORCID: and Jere, Khuzwayo C. (2023) 'Acute rotavirus infection is associated with the induction of circulating memory CD4 + T cell subsets'. Scientific Reports, Vol 13, Issue 1, e9001.

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Strong CD4+ T cell-mediated immune protection following rotavirus infection has been observed in animal models, but its relevance in humans remains unclear. Here, we characterized acute and convalescent CD4+ T cell responses in children who were hospitalized with rotavirus-positive and rotavirus-negative diarrhoea in Blantyre, Malawi. Children presenting with laboratory-confirmed rotavirus infection had higher proportions of effector and central memory T helper 2 cells during acute infection i.e., at disease presentation compared to convalescence, 28 days post-infection defined by a follow-up 28 days after acute infection. However, circulating cytokine-producing (IFN-γ and/or TNF-α) rotavirus-specific VP6-specific CD4+ T cells were rarely detectable in children with rotavirus infection at both acute and convalescent stages. Moreover, following whole blood mitogenic stimulation, the responding CD4+ T cells were predominantly non-cytokine producers of IFN-γ and/or TNF-α. Our findings demonstrate limited induction of anti-viral IFN-γ and/or TNF-α-producing CD4+ T cells in rotavirus-vaccinated Malawian children following the development of laboratory-confirmed rotavirus infection.

Item Type: Article
Subjects: WC Communicable Diseases > Virus Diseases > General Virus Diseases > WC 500 Virus diseases (General or not elsewhere classified)
WS Pediatrics > By Age Groups > WS 440 Preschool child
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW)
Digital Object Identifer (DOI):
SWORD Depositor: JISC Pubrouter
Depositing User: JISC Pubrouter
Date Deposited: 05 Jun 2023 11:50
Last Modified: 06 Jun 2023 09:07


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