Marriott, Amy, Dagley, Jessica, Hegde, Shree ORCID: https://orcid.org/0000-0002-6673-7780, Steven, Andrew, Fricks, C., DiCosty, U., Mansour, A., Campbell, E. J., Wilson, C. M., Gusovsky, F., Ward, S, Hong, W. D., O'Neill, P., Moorhead, A., McCall, S., McCall, J. W., Taylor, Mark ORCID: https://orcid.org/0000-0003-3396-9275 and Turner, Joseph ORCID: https://orcid.org/0000-0002-2185-5476 (2023) 'Dirofilariasis mouse models for heartworm preclinical research'. Frontiers in Microbiology, Vol 14, e1208301.
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Abstract
Introduction: Dirofilariasis, including heartworm disease, is a major emergent veterinary parasitic infection and a human zoonosis. Currently, experimental infections of cats and dogs are used in veterinary heartworm preclinical drug research.
Methods: As a refined alternative in vivo heartworm preventative drug screen, we assessed lymphopenic mouse strains with ablation of the interleukin-2/7 common gamma chain (γc) as susceptible to the larval development phase of Dirofilaria immitis.
Results: Non-obese diabetic (NOD) severe combined immunodeficiency (SCID)γc−/− (NSG and NXG) and recombination-activating gene (RAG)2−/−γc−/− mouse strains yielded viable D. immitis larvae at 2–4 weeks post-infection, including the use of different batches of D. immitis infectious larvae, different D. immitis isolates, and at different laboratories. Mice did not display any clinical signs associated with infection for up to 4 weeks. Developing larvae were found in subcutaneous and muscle fascia tissues, which is the natural site of this stage of heartworm in dogs. Compared with in vitro-propagated larvae at day 14, in vivo-derived larvae had completed the L4 molt, were significantly larger, and contained expanded Wolbachia endobacteria titres. We established an ex vivo L4 paralytic screening system whereby assays with moxidectin or levamisole highlighted discrepancies in relative drug sensitivities in comparison with in vitro-reared L4 D. immitis. We demonstrated effective depletion of Wolbachia by 70%−90% in D. immitis L4 following 2- to 7-day oral in vivo exposures of NSG- or NXG-infected mice with doxycycline or the rapid-acting investigational drug, AWZ1066S. We validated NSG and NXG D. immitis mouse models as a filaricide screen by in vivo treatments with single injections of moxidectin, which mediated a 60%−88% reduction in L4 larvae at 14–28 days.
Discussion: Future adoption of these mouse models will benefit end-user laboratories conducting research and development of novel heartworm preventatives via increased access, rapid turnaround, and reduced costs and may simultaneously decrease the need for experimental cat or dog use.
Item Type: | Article | ||||
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Subjects: | QV Pharmacology > QV 38 Drug action. QV Pharmacology > QV 4 General works QW Microbiology and Immunology > Bacteria > QW 150 Proteobacteria. Rickettsiaceae, Wolbachia WB Practice of Medicine > Therapeutics > WB 340 Drug Administration |
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Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology | ||||
Digital Object Identifer (DOI): | https://doi.org/10.3389/fmicb.2023.1208301 | ||||
Depositing User: | Clare Bennett | ||||
Date Deposited: | 03 Jul 2023 10:39 | ||||
Last Modified: | 01 Aug 2024 08:28 | ||||
URI: | https://archive.lstmed.ac.uk/id/eprint/22741 |
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