The identification of enteric fever-specific antigens for population based serosurveillance

Mylona, Elli, Hefele, Lisa, Tran Vu Thieu, Nga, Trinh Van, Tan, Nguyen Ngoc Minh, Chau, Tran Tuan, Anh, Karkey, Abhilasha, Dongol, Sabina, Basnyat, Buddha, Voong Vinh, Phat, Ho Ngoc Dan, Thanh, Russell, Paula, Charles, Richelle C, Parry, Christopher and Baker, Stephen (2023) 'The identification of enteric fever-specific antigens for population based serosurveillance'. The Journal of Infectious Diseases. (In Press)

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Abstract

Background
Enteric fever, caused by Salmonella enterica serovars Typhi and Paratyphi A, is a major public health problem in low and middle-income countries. Moderate sensitivity and scalability of current methods likely underestimate enteric fever burden. Determining the serological responses to organism-specific antigens may improve incidence measures.

Methods
Plasma samples were collected from blood culture-confirmed enteric fever patients, blood culture-negative febrile patients over the course of three months and afebrile community controls. A panel of 17 Salmonella Typhi and Paratyphi A antigens was purified and used to determine antigen-specific antibody responses by indirect ELISAs.

Results
The antigen-specific longitudinal antibody responses were comparable between enteric fever patients, patients with blood culture-negative febrile controls, and afebrile community controls for most antigens. However, we found that IgG responses against STY1479 (YncE), STY1886 (CdtB), STY1498 (HlyE) and the serovar-specific O2 and O9 antigens were greatly elevated over a three-month follow up period in S. Typhi/S. Paratyphi A patients compared to controls, suggesting seroconversion.

Conclusions
We identified a set of antigens as good candidates to demonstrate enteric fever exposure. These targets can be used in combination to develop more sensitive and scalable approaches to enteric fever surveillance and generate invaluable epidemiological data for informing vaccine policies.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 573 Antigens WC Communicable Diseases > Infection. Bacterial Infections > Enteric Infections > WC 260 Enterobacteriaceae and other enteric infections
Faculty: Department:	Education
Digital Object Identifer (DOI):	https://doi.org/10.1093/infdis/jiad242
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	14 Jul 2023 07:44
Last Modified:	16 Feb 2024 14:00
URI:	https://archive.lstmed.ac.uk/id/eprint/22799

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