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Knockdown resistance allele L1014F introduced by CRISPR/Cas9 is not associated with altered vector competence of Anopheles gambiae for o’nyong nyong virus

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Kay, Grant Alistair, Patterson, Edward I., Hughes, Grant ORCID: https://orcid.org/0000-0002-7567-7185, Lord, Jennifer and Reimer, Lisa ORCID: https://orcid.org/0000-0002-9711-4981 (2023) 'Knockdown resistance allele L1014F introduced by CRISPR/Cas9 is not associated with altered vector competence of Anopheles gambiae for o’nyong nyong virus'. PLoS ONE, Vol 18, Issue 8, e0288994.

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Official URL: https://doi.org/10.1371/journal.pone.0288994

Abstract

Knockdown resistance (kdr) alleles conferring resistance to pyrethroid insecticides are widespread amongst vector populations. Previous research has suggested that these alleles are associated with changes in the vector competence of mosquitoes for arboviruses and Plasmodium, however non-target genetic differences between mosquito strains may have had a confounding effect. Here, to minimise genetic differences, the laboratory Anopheles gambiae Kisumu strain was compared to a CRISPR/Cas9 homozygous kdr L1014F mutant Kisumu-kdr line in order to examine associations with vector competence for o’nyong nyong virus (ONNV). Mosquitoes were infected using either blood feeds or intrathoracic microinjections. There were no significant differences in the prevalence of virus in mosquito body parts between kdr mutant and wildtype lines from either oral or intrathoracic injection routes. The ONNV titre was significantly higher in the legs of the wildtype strain at 7dpi following intrathoracic microinjection, but no other significant differences in viral titre were detected. ONNV was not detected in the saliva of mosquitoes from either strain. Our findings from per os infections suggest that the kdr L1014F allele is not associated with altered infection prevalence for ONNV, a key component of vector competence.

Item Type:

Article

Subjects:

QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors

Repository link:

Item title	Item URI
Dataset for the article: Knockdown resistance allele L1014F introduced by CRISPR/Cas9 is not associated with altered vector competence of Anopheles gambiae for o’nyong nyong virus	https://archive.lstmed.ac.uk/id/eprint/22941

Faculty: Department:

Biological Sciences > Vector Biology Department

Digital Object Identifer (DOI):

https://doi.org/10.1371/journal.pone.0288994

SWORD Depositor:

JISC Pubrouter

Depositing User:

JISC Pubrouter

Date Deposited:

11 Aug 2023 08:40

Last Modified:

11 Aug 2023 09:32