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Hawkes, Nicola J. and Hemingway, Janet 
ORCID: https://orcid.org/0000-0002-3200-7173
(2002)
'Analysis of the promoters for the beta-esterase genes associated with insecticide resistance in the mosquito Culex quinquefasciatus'. Biochimica Et Biophysica Acta-Gene Structure and Expression, Vol 1574, Issue 1, pp. 51-62.
Abstract
Resistance to organophosphorus (OP) insecticides in the mosquito Culex quinquefasciatus is primarily due to the amplification and overexpression of non-specific esterases. Co-amplification of two esterase genes, estalpha2(1) and estbeta2(1), is the most common resistance genotype. In both resistant and susceptible mosquitoes the alpha- and beta-esterase genes are oriented in a head-to-head arrangement, the intergenic sequences containing promoter elements for the divergent transcription of both esterases. Transient transfection of luciferase reporter gene constructs into a C quinquefasciatus cell line was used to study these promoters. A control vector was constructed with the strong Drosophila actin 5c promoter driving expression of beta-galactosidase. The beta-esterase promoters from both insecticide resistant and -susceptible insects were highly active in directing luciferase expression. Transfections with panels of deletions revealed several regions where binding sites for positive and negative regulatory elements are located, and candidate transcription factor sites have been identified. Gel shift assays have identified one DNA-protein interaction that is stronger with the resistant than with the equivalent but slightly altered susceptible sequence. The arthropod initiator site TCAGT 135bp upstream of the ATG in both beta-esterase promoters is essential for transcription initiation, but a putative TATA box is not involved. (C) 2002 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | β-Esterase; Promoter analysis; Arthropod initiator; Culex quinquefasciatus |
| Subjects: | QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance. QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 530 Culex QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/S0167-4781(01)00344-X |
| Depositing User: | Sarah Lewis-Newton |
| Date Deposited: | 20 Aug 2013 10:18 |
| Last Modified: | 06 Feb 2018 13:03 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/2311 |
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