Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae

Vontas, John, Hejazi, M. J., Hawkes, Nicola J., Cosmidis, N., Loukas, M. and Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 (2002) 'Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae'. Insect Molecular Biology, Vol 11, Issue 4, pp. 329-336.

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Abstract

A 2.2-kb full length cDNA containing an ORF encoding a putative acetylcholinesterase (AChE) precursor of 673 amino acid residues was obtained by a combined degenerate PCR and RACE strategy from an organophosphate-susceptible Bactrocera oleae strain. A comparison of cDNA sequences of individual insects from susceptible and resistant strains, coupled with an enzyme inhibition assay with omethoate, indicated a novel glycine-serine substitution (G488S), at an amino acid residue which is highly conserved across species (13396 of Torpedo californica AChE), as a likely cause of AChE insensitivity. This mutation was also associated with a 35-40% reduction in AChE catalytic efficiency. The I199V substitution, which confers low levels of resistance in Drosophila, was also present in B. oleae (I214V) and in combination with G488S produced up to a 16-fold decrease in insecticide sensitivity. This is the first agricultural pest where resistance has been associated with an alteration in AChE, which arises from point mutations located within the active site gorge of the enzyme.

Item Type:	Article
Additional Information:	The definitive version is available at www3.interscience.wiley.com
Subjects:	QU Biochemistry > Enzymes > QU 135 Enzymes QU Biochemistry > Genetics > QU 470 Genetic structures QX Parasitology > Insects. Other Parasites > QX 505 Diptera QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1046/j.1365-2583.2002.00343.x
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	22 Feb 2013 11:53
Last Modified:	06 Feb 2018 13:03
URI:	https://archive.lstmed.ac.uk/id/eprint/2319

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