Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae

Blandin, S. A., Wang-Sattler, R., Lamacchia, M., Gagneur, J., Lycett, Gareth ORCID: https://orcid.org/0000-0002-2422-053X, Ning, Y., Levashina, E. A. and Steinmetz, L. M. (2009) 'Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae'. Science, Vol 326, Issue 5949, pp. 147-150.

Full text not available from this repository.

Abstract

The ability of Anopheles gambiae mosquitoes to transmit Plasmodium parasites is highly variable between individuals. However, the genetic basis of this variability has remained unknown. We combined genome-wide mapping and reciprocal allele-specific RNA interference (rasRNAi) to identify the genomic locus that confers resistance to malaria parasites and demonstrated that polymorphisms in a single gene encoding the antiparasitic thioester-containing protein 1 (TEPl) explain a substantial part of the variability in parasite killing. The link between TEP1 alleles and resistance to malaria may offer new tools for controlling malaria transmission. The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles.

Item Type:	Article
Uncontrolled Keywords:	protozoal protein thioester containing protein 1 unclassified drug allele gene expression genetic analysis genomics malaria mosquito parasite resistance phenotype protozoan RNA Anopheles gambiae article controlled study female gene frequency gene identification gene locus gene mapping genetic linkage genetic variability mouse nonhuman parasite transmission Plasmodium priority journal quantitative trait RNA interference single nucleotide polymorphism Plasmodium parasites
Subjects:	QX Parasitology > Protozoa > QX 135 Plasmodia WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QU Biochemistry > Genetics > QU 475 Genetic processes QU Biochemistry > Genetics > QU 470 Genetic structures QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1126/science.1175241
Depositing User:	Users 183 not found.
Date Deposited:	22 Jun 2010 09:27
Last Modified:	22 Aug 2019 11:03
URI:	https://archive.lstmed.ac.uk/id/eprint/232

Statistics

View details

Actions (login required)

Edit Item