Male vitellogenin regulates gametogenesis through a testis‐enriched big protein in Chrysopa pallens

Liu, Xiao‐ping, Liu, Chang‐yan, Feng, Yan‐jiao, Guo, Xing‐kai, Zhang, Li‐sheng, Wang, Meng‐qing, Li, Yu‐yan, Zeng, Fan‐rong, Nolan, Tony ORCID: https://orcid.org/0000-0002-2982-8333 and Mao, Jian‐jun (2023) 'Male vitellogenin regulates gametogenesis through a testis‐enriched big protein in Chrysopa pallens'. Insect Molecular Biology, Vol 33, Issue 1, pp. 17-28.

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Abstract

In insects, vitellogenin (Vg) is generally viewed as a female‐specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg‐deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole‐organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis‐related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis‐enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction.

Item Type:	Article
Subjects:	QU Biochemistry > Proteins. Amino Acids. Peptides > QU 55 Proteins QX Parasitology > Insects. Other Parasites > QX 500 Insects QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1111/imb.12873
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	16 Oct 2023 09:50
Last Modified:	06 Feb 2024 12:04
URI:	https://archive.lstmed.ac.uk/id/eprint/23208

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