ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming

Menzies, Stefanie ORCID: https://orcid.org/0000-0002-9273-9296, Arinto-Garcia, Raquel, Amorim, Fernanda Gobbi, Cardoso, Iara Aimê, Abada, Camille, Crasset, Thomas, Durbesson, Fabien, Edge, Rebecca J., El-Kazzi, Priscila, Hall, Sophie, Redureau, Damien, Stenner, Richard, Boldrini-França, Johara, Sun, Huan, Roldão, António, Alves, Paula M., Harrison, Robert, Vincentelli, Renaud, Berger, Imre, Quinton, Loïc, Casewell, Nicholas R. and Schaffitzel, Christiane (2023) 'ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming'. Toxins, Vol 15, Issue 12, e673.

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Abstract

Snakebite envenoming can be a life-threatening medical emergency that requires prompt medical intervention to neutralise the effects of venom toxins. Each year up to 138,000 people die from snakebites and threefold more victims suffer life-altering disabilities. The current treatment of snakebite relies solely on antivenom—polyclonal antibodies isolated from the plasma of hyperimmunised animals—which is associated with numerous deficiencies. The ADDovenom project seeks to deliver a novel snakebite therapy, through the use of an innovative protein-based scaffold as a next-generation antivenom. The ADDomer is a megadalton-sized, thermostable synthetic nanoparticle derived from the adenovirus penton base protein; it has 60 high-avidity binding sites to neutralise venom toxins. Here, we outline our experimental strategies to achieve this goal using state-of-the-art protein engineering, expression technology and mass spectrometry, as well as in vitro and in vivo venom neutralisation assays. We anticipate that the approaches described here will produce antivenom with unparalleled efficacy, safety and affordability.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.3390/toxins15120673
Depositing User:	Clare O'Neill
Date Deposited:	04 Dec 2023 13:33
Last Modified:	04 Dec 2023 13:33
URI:	https://archive.lstmed.ac.uk/id/eprint/23604

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