Developing Wolbachia-Infected Cell Lines from Insects.

Madhav, Mukund and James, Peter (2023) 'Developing Wolbachia-Infected Cell Lines from Insects.' in: Wolbachia Methods and Protocols, 2739, Springer Link, pp. 137-145.

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Abstract

Wolbachia, an intracellular bacterium of arthropods, is an ideal candidate for use in the biological control of insect pests. The inability of Wolbachia to grow in the extracellular environment requires maintenance in live insects or insect cell cultures. Growing and adapting high-density Wolbachia in the targeted host cells improves the possibility of cross-species transinfection. Here, we describe a method for the establishment of a primary cell culture from insect embryos and its transinfection with Wolbachia.

Item Type:	Book Section
Subjects:	QW Microbiology and Immunology > Bacteria > QW 131 Gram-negative bacteria. QX Parasitology > QX 20 Research (General)
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1007/978-1-0716-3553-7_8
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	08 Feb 2024 11:36
Last Modified:	08 Feb 2024 11:36
URI:	https://archive.lstmed.ac.uk/id/eprint/23661

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