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Lagnika, Hamirath O., Medjigbodo, Adandé A., Djihinto, Oswald Y., Saïzonou, Helga M., Mousse, Wassiyath A., Akoton, Romaric, Djossou, Laurette, Vodounkpe, Doris N., Lagnika, Latifou and Djogbenou, Luc (2024) 'Beninese Plant Extracts with Antiplasmodial Activity Select New Allele Variants Msp1 and Msp2 in Plasmodium falciparum'. Journal of Parasitology Research, Vol 2024, p. 9980715.
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Abstract
Background. Natural medicinal products are commonly used as a remedy against malaria infections in African populations and have become a major source of information for the screening of new and more effective antiplasmodial molecules. Therefore, in vitro studies are needed to validate the efficacy of these medicinal products and to explore the potential effects of such drugs on the genetic diversity of Plasmodium falciparum. The current study has investigated the impact of some Beninese plant extracts with antiplasmodial activity on the genetic diversity of P. falciparum.
Method. Five (5) ethanolic plant extracts (Dissotis rotundifolia, Ehretia cymosa Thonn, Hibiscus surattensis L., Cola millenii K. Shum, and Costus afer Ker Gawl) and a compound extracted from Ehretia cymosa Thonn (encoded CpE2) were tested against asexual stage parasites of a culture-adapted strain of P. falciparum. Subsequently, the P. falciparum Msp1 and Msp2 markers were genotyped, and the number of allelic variants and the multiplicity of infection (MOI) were compared between drug-exposed and unexposed parasites.
Results. All plant extracts have shown inhibitory activity against asexual P. falciparum and selected new allelic variants of the Msp1 and Msp2 genes compared to unexposed parasites. The newly selected allelic variants were K1_100bp and RO33_300bp of the Msp1 gene and FC27_150bp, FC27_300bp, FC27_400bp, and FC27_600bp of the Msp2 gene. However, there was no significant difference in MOI between drug-exposed and unexposed parasites.
Conclusion. Our study highlights a source for the selection of new Msp1 and Msp2 alleles after exposure to antimalarial drugs. These findings pave the way for further studies investigating the true roles of these newly selected alleles in P. falciparum.
| Item Type: | Article |
|---|---|
| Subjects: | QV Pharmacology > Drug Standardization. Pharmacognosy. Medicinal Plants > QV 766 Medicinal plants (General) QX Parasitology > Protozoa > QX 135 Plasmodia QX Parasitology > QX 20 Research (General) |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1155/2024/9980715 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 10 Apr 2024 08:50 |
| Last Modified: | 16 Apr 2024 13:23 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/24299 |
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