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McGalliard, Rachel, Muhamadali, Howbeer, AlMasoud, Najla, Haldenby, Sam, Romero-Soriano, Valeria, Allman, Ellie, Xu, Yun, Roberts, Adam 
ORCID: https://orcid.org/0000-0002-0760-3088, Paterson, Steve, Carrol, Enitan D and Goodacre, Royston
(2024)
'Bacterial discrimination by Fourier transform infrared spectroscopy, MALDI-mass spectrometry and whole-genome sequencing'. Future Microbiology.
(In Press)
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mcgalliard-et-al-2024-bacterial-discrimination-by-fourier-transform-infrared-spectroscopy-maldi-mass-spectrometry-and A robets 29.04.2024.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (3MB) | Preview |
Abstract
Aim: Proof-of-concept study, highlighting the clinical diagnostic ability of FT-IR compared with MALDI-TOF MS, combined with WGS. Materials & methods: 104 pathogenic isolates of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes and Staphylococcus aureus were analyzed. Results: Overall prediction accuracy was 99.6% in FT-IR and 95.8% in MALDI-TOF-MS. Analysis of N. meningitidis serogroups was superior in FT-IR compared with MALDI-TOF-MS. Phylogenetic relationship of S. pyogenes was similar by FT-IR and WGS, but not S. aureus or S. pneumoniae. Clinical severity was associated with the zinc ABC transporter and DNA repair genes in S. pneumoniae and cell wall proteins (biofilm formation, antibiotic and complement permeability) in S. aureus via WGS. Conclusion: FT-IR warrants further clinical evaluation as a promising diagnostic tool.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Genetics > QU 550 Genetic techniques. PCR. Chromosome mapping QW Microbiology and Immunology > QW 50 Bacteria (General). Bacteriology. Archaea |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.2217/fmb-2024-0043 |
| Depositing User: | Clare O'Neill |
| Date Deposited: | 01 May 2024 14:22 |
| Last Modified: | 01 May 2024 14:22 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/24472 |
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