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Praulins, Giorgio, Murphy-Fegan, Annabel, Gillespie, Jack, Mechan, Frank, Gleave, Katherine and Lees, Rosemary (2024) 'Unpacking WHO and CDC Bottle Bioassay Methods: A Comprehensive Literature Review and Protocol Analysis Revealing Key Outcome Predictors'. Gates Open Research, Vol 8, e56.
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77628051-b4a7-4e8e-948f-5b68600d6f6a_15433_-_rosemary_lees.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Background
Resistance monitoring is a key element in controlling vector-borne diseases. The World Health Organization (WHO) and Centres for Disease Control and Prevention (CDC) have each developed bottle bioassay methods for determining insecticide susceptibility in mosquito vectors which are used globally.
Methods
This study aimed to identify variations in bottle bioassay methodologies and assess the potential impact on the data that is generated. Our approach involved a systematic examination of existing literature and protocols from WHO and CDC, with a focus on the specifics of reported methodologies, variation between versions, and reported outcomes. Building on this, we experimentally evaluated the impact of several variables on bioassay results.
Results
Our literature review exposed a significant inconsistency in the how bioassay methods are reported, hindering reliable interpretation of data and the ability to compare results between studies. The experimental research provided further insight by specifically identifying two key factors that influence the outcomes of bioassays: mosquito dry weight and relative humidity (RH). This finding not only advances our comprehension of these assays but also underscores the importance of establishing precisely defined methodologies for resistance monitoring. The study also demonstrates the importance of controlling bioassay variables, noting the significant influence of wing length, as an indicator of mosquito size, on mortality rates in standardized bioassays.
Conclusions
Generating data with improved protocol consistency and precision will not only deepen our understanding of resistance patterns but also better inform vector control measures. We call for continued research and collaboration to refine and build consensus on bioassay techniques, to help bolster the global effort against vector-borne diseases like malaria.
| Item Type: | Article | ||||
|---|---|---|---|---|---|
| Subjects: | QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Health Administration and Organization > WA 530 International health administration |
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| Faculty: Department: | Biological Sciences > Vector Biology Department | ||||
| Digital Object Identifer (DOI): | https://doi.org/10.12688/gatesopenres.15433.1 | ||||
| SWORD Depositor: | JISC Pubrouter | ||||
| Depositing User: | JISC Pubrouter | ||||
| Date Deposited: | 20 Jun 2024 10:54 | ||||
| Last Modified: | 26 Jun 2024 12:55 | ||||
| URI: | https://archive.lstmed.ac.uk/id/eprint/24755 |
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