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Tingwei, Cai, Nadal-Jimenez, Pol, Yuanyuan, Gao, Hiroshi, Arai, Chengyue, Li, Chunyan, Su, King, Kayla, Shun, He, Jianhong, Li, Hurst, Gregory and Wan, Hu (2024) 'Insecticide susceptibility in a planthopper pest increases following inoculation with cultured Arsenophonus'. The ISME Journal. (In Press)
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Abstract
Facultative vertically transmitted symbionts are a common feature of insects that determine many aspects of their hosts’ phenotype. Our capacity to understand and exploit these symbioses is commonly compromised by the microbes unculturability and consequent lack of genetic tools, an impediment of particular significance for symbioses of pest and vector species. Previous work had established that insecticide susceptibility of the economically important pest of rice, the brown planthopper Nilaparvata lugens, was higher in field-collected lineages that carry Ca. Arsenophonus nilaparvatae. We established Ca. A. nilaparvatae into cell-free culture and used this to establish the complete closed genome of the symbiont. We transformed the strain to express GFP and reintroduced it to N. lugens to track infection in vivo. The symbiont established vertical transmission, generating a discrete infection focus towards the posterior pole of each N. lugens oocyte. This infection focus was retained in early embryogenesis before transition to a diffuse somatic infection in late N. lugens embryos and nymphs. We additionally generated somatic infection in novel host species, but these did not establish vertical transmission. Transinfected planthopper lines acquired the insecticide sensitivity trait, with associated downregulation of the P450 xenobiotic detoxification system of the host. Our results causally establish the role of the symbiont in increasing host insecticide sensitivity with implications for insecticide use and stewardship. Further, the culturability and transformation of this intracellular symbiont, combined with its ease of reintroduction to planthopper hosts, enables novel approaches both for research into symbiosis and into control of insect pest species.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > Viruses > QW 162 Insect viruses QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1093/ismejo/wrae194 |
| Depositing User: | Rachael Kedge |
| Date Deposited: | 14 Oct 2024 07:30 |
| Last Modified: | 15 Oct 2024 09:04 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/25465 |
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