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Garcia-Guerra, Antonio, Sathyaprakash, Chaitra, de Jong, Olivier G, Lim, Wooi F, Vader, Pieter, El Andaloussi, Samir, Bath, Jonathan, Reiné, Jesús, Aoki, Yoshitsugu, Turberfield, Andrew J, Wood, Matthew J A and Rinaldi, Carlo (2025) 'Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs'. Nucleic Acids Research, Vol 53, Issue 2.
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gkaf016.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Nucleic acid nanostructures offer unique opportunities for biomedical applications due to their sequence-programmable structures and functions, which enable the design of complex responses to molecular cues. Control of the biological activity of therapeutic cargoes based on endogenous molecular signatures holds the potential to overcome major hurdles in translational research: cell specificity and off-target effects. Endogenous microRNAs (miRNAs) can be used to profile cell type and cell state, and are ideal inputs for RNA nanodevices. Here, we present CRISPR MiRAGE (miRNA-activated genome editing), a tool comprising a dynamic single-guide RNA that senses miRNA complexed with Argonaute proteins and controls downstream CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) activity based on the detected miRNA signature. We study the operation of the miRNA-sensing single-guide RNA and attain muscle-specific activation of gene editing through CRISPR MiRAGE in models of Duchenne muscular dystrophy. By enabling RNA-controlled gene editing activity, this technology creates opportunities to advance tissue-specific CRISPR treatments for human diseases.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics. W General Medicine. Health Professions > W 20.5 Biomedical research |
| Faculty: Department: | Clinical Sciences & International Health > Clinical Sciences Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1093/nar/gkaf016 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 20 Feb 2025 12:11 |
| Last Modified: | 20 Feb 2025 12:11 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/26050 |
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