Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors

Petit, Marine J., Flory, Charlotte, Gu, Quan, Fares, Mazigh, Lamont, Douglas, Score, Alan, Davies, Kelsey, Bell-Sakyi, Lesley, Scaturro, Pietro, Brennan, Benjamin and Kohl, Alain (2025) 'Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors'. Nature Communications, Vol 16, Issue 1, p. 4732.

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Abstract

The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WC Communicable Diseases > Rickettsiaceae Infections. Chlamydiaceae Infections > WC 600 Rickettsiaceae infections. Chlamydiaceae infections. Tick-borne diseases
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1038/s41467-025-59565-w
Depositing User:	Clare O'Neill
Date Deposited:	03 Jun 2025 09:42
Last Modified:	03 Jun 2025 09:42
URI:	https://archive.lstmed.ac.uk/id/eprint/26706

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