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Diaz-Albiter, Hector, Sant'Anna, Mauricio R.V., Genta, F. A. and Dillon, Rod (2012) 'Reactive Oxygen Species-mediated Immunity against Leishmania mexicana and Serratia marcescens in the Phlebotomine Sand Fly Lutzomyia longipalpis'. Journal of Biological Chemistry, Vol 287, Issue 28, pp. 23995-24003.
Full text not available from this repository.Abstract
Phlebotomine sand flies are the vectors of medically important Leishmania. The Leishmania protozoa reside in the sand fly gut, but the nature of the immune response to the presence of Leishmania is unknown. Reactive oxygen species (ROS) are a major component of insect innate immune pathways regulating gut-microbe homeostasis. Here we show that the concentration of ROS increased in sand fly midguts after they fed on the insect pathogen Serratia marcescens but not after feeding on the Leishmania that uses the sand fly as a vector. Moreover, the Leishmania is sensitive to ROS either by oral administration of ROS to the infected fly or by silencing a gene that expresses a sand fly ROS-scavenging enzyme. Finally, the treatment of sand flies
with an exogenous ROS scavenger (uric acid) altered the gut
microbial homeostasis, led to an increased commensal gut
microbiota, and reduced insect survival after oral infection with S. marcescens. Our study demonstrates a differential response of the sand fly ROS system to gut microbiota, an insect pathogen, and the Leishmania that utilize the sand fly as a vehicle for transmission between mammalian hosts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Innate Immunity Insect immunity Leishmania Parasitology Reactive Oxygen Species (ROS) Lutzomyia Sand Fly |
| Subjects: | QW Microbiology and Immunology > Immunity by Type > QW 551 Acquired immunity. Artificial immunity QW Microbiology and Immunology > Immunity by Type > QW 563 Local immunity QX Parasitology > Insects. Other Parasites > QX 505 Diptera WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1074/jbc.M112.376095 |
| Depositing User: | Users 183 not found. |
| Date Deposited: | 20 Jul 2012 13:36 |
| Last Modified: | 06 Feb 2018 13:04 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/2865 |
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