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Harrison, Robert, Richards, A., Laing, Gavin and Theakston, R.David G. (2002) 'Simultaneous GeneGun immunisation with plasmids encoding antigen and GM-CSF: significant enhancement of murine antivenom IgG1 titres'. Vaccine, Vol 20, Issue 13-14, pp. 1702-1706.
Full text not available from this repository.Abstract
GeneGun DNA immunisation is a potent means of inducing antibody-dominant immune responses that we are exploiting to generate venom toxin-specific antibodies to improve the therapy of systemic envenoming by snakes. Here, we report that mice immunised with DNA encoding the carboxyl domain (JD9) of a haemorrhagic Zn metalloprotease (Jararhagin) in venom of the South American pit viper, Bothrops jararaca, and a plasmid expressing murine cytokine granulocyte/macrophage-colony stimulating factor (GM-CSF) raised significantly higher antigen-specific IgGl titres than mice immunised with JD9 DNA alone. Serological responses to GeneGun JD9 DNA immunisation were shown to be dominated by IgGl, an IgG subclass associated with T lymphocyte helper 2 (Th2) immune responses. Further significant enhancement of JD9-specific IgGl titres was achieved by increasing the number of immunisations. This report illustrates that DNA immunisation protocols to achieve high-titre, venom toxin-specific antibody production are well advanced and encourage the development of a DNA-based approach to antivenom production. (C) 2002 Elsevier Science Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Genetics > QU 470 Genetic structures QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles |
| Faculty: Department: | Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/s0264-410x(02)00026-9 |
| Depositing User: | Martin Chapman |
| Date Deposited: | 22 Oct 2013 13:01 |
| Last Modified: | 06 Feb 2018 13:05 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/2933 |
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