Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi

Suwan, N., Wilkinson, M. C., Crampton, J. M. and Bates, Paul (2002) 'Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi'. Insect Molecular Biology, Vol 11, Issue 3, pp. 223-232.

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Abstract

Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium, berghei-infected and uninfected mosquitoes.

Item Type:	Article
Subjects:	QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA. QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.7 RNA QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1046/j.1365-2583.2002.00329.x
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	10 Jul 2013 10:12
Last Modified:	17 Jul 2020 10:58
URI:	https://archive.lstmed.ac.uk/id/eprint/2990

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