Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica

Morphew, Russell M, Eccleston, Neil, Wilkinson, Toby J, McGarry, John, Perally, Samirah, Prescott, Mark, Ward, Deborah, Williams, Diana, Paterson, Steve, Raman, M, Ravikumar, G, Khalid Saifullah, M, Abbas Abidi, S M, McVeigh, Paul, Maule, Aaron G, Brophy, Peter M and LaCourse, James ORCID: https://orcid.org/0000-0001-9261-7136 (2012) 'Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica'. Journal of Proteome Research, Vol 11, Issue 12, pp. 5876-5889.

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Abstract

Fasciolosis is an important foodborne, zoonotic disease of livestock and humans, with global annual health and economic losses estimated at several billion US$. Fasciola hepatica is the major species in temperate regions, while F. gigantica dominates in the tropics. In the absence of commercially available vaccines to control fasciolosis, increasing reports of resistance to current chemotherapeutic strategies and the spread of fasciolosis into new areas, new functional genomics approaches are being used to identify potential new drug targets and vaccine candidates. The glutathione transferase (GST) superfamily is both a candidate drug and vaccine target. This study reports the identification of a putatively novel Sigma class GST, present in a water-soluble cytosol extract from the tropical liver fluke F. gigantica. The GST was cloned and expressed as an enzymically active recombinant protein. This GST shares a greater identity with the human schistosomiasis GST vaccine currently at Phase II clinical trials than previously discovered F. gigantica GSTs, stimulating interest in its immuno-protective properties. In addition, in silico analysis of the GST superfamily of both F. gigantica and F. hepatica has revealed an additional Mu class GST, Omega class GSTs, and for the first time, a Zeta class member.

Item Type:	Article
Uncontrolled Keywords:	Fasciola gigantica, glutathione transferase, Sigma, Omega, Zeta, Mu
Subjects:	QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA. QU Biochemistry > Proteins. Amino Acids. Peptides > QU 68 Peptides QX Parasitology > Helminths. Annelida > QX 365 Fasciola
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1021/pr300654w
Depositing User:	Mary Creegan
Date Deposited:	16 Jul 2014 10:38
Last Modified:	06 Feb 2018 13:07
URI:	https://archive.lstmed.ac.uk/id/eprint/3806

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