Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR

Adams, Emily ORCID: https://orcid.org/0000-0002-0816-2835, Gomez, Maria Adelaida, Scheske, Laura, Rios, Ruby, Marquez, Ricardo, Cossio, Alexandra, Albertini, Audrey, Schallig, Henk and Saravia, Nancy Gore (2014) 'Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR'. Parasitology, Vol 141, Issue 14, pp. 1891-1897.

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Abstract

Summary: Variation in clinical accuracy of molecular diagnostic methods for cutaneous leishmaniasis (CL) is commonly observed depending on the sample source, the method of DNA recovery and the molecular test. Few attempts have been made to compare these variables. Two swab and aspirate samples from lesions of patients with suspected CL (n = 105) were evaluated alongside standard diagnosis by microscopic detection of amastigotes or culture of parasites from lesion material. Three DNA extraction methods were compared: Qiagen on swab and aspirate specimens, Isohelix on swabs and Boil/Spin of lesion aspirates. Recovery of Leishmania DNA was evaluated for each sample type by real-time polymerase chain reaction detection of parasitic 18S rDNA, and the diagnostic accuracy of the molecular method determined. Swab sampling combined with Qiagen DNA extraction was the most efficient recovery method for Leishmania DNA, and was the most sensitive (98%; 95% CI: 91–100%) and specific (84%; 95% CI: 64–95%) approach. Aspirated material was less sensitive at 80% (95% CI: 70–88%) and 61% (95% CI: 50–72%) when coupled to Qiagen or Boil-Spin DNA extraction, respectively. Swab sampling of lesions was painless, simple to perform and coupled with standardized DNA extraction enhances the feasibility of molecular diagnosis of CL.

Item Type:	Article
Additional Information:	Special Issue - Symposia of the British Society for Parasitology Volume 52 Advances in diagnostics for parasitic diseases.
Subjects:	WR Dermatology > WR 141 Examination. Diagnosis. Diagnostic methods. Monitoring WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1017/S0031182014001280
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	30 Oct 2014 12:14
Last Modified:	15 Jun 2018 10:30
URI:	https://archive.lstmed.ac.uk/id/eprint/4505

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