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Dawson, Emily M, Sousa-Figueiredo, J.C., Kabatereine, Narcis B, Doenhoff, Michael J and Stothard, Russell 
ORCID: https://orcid.org/0000-0002-9370-3420
(2013)
'Intestinal schistosomiasis in pre school-aged children of Lake Albert, Uganda: diagnostic accuracy of a rapid test for detection of anti-schistosome antibodies.'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 107, Issue 10, pp. 639-647.
Abstract
BACKGROUND
A sensitive and reliable rapid diagnostic test (RDT) which should have comparable diagnostic performance against reference host serological methods is urgently needed for use in point-of-care (POC) diagnosis of intestinal schistosomiasis in pre school-aged children.
METHODS
The diagnostic accuracy of a RDT incorporating Schistosoma mansoni cercarial transformation fluid (SmCTF) for anti-schistosome antibody detection was evaluated with serum samples from a cohort of children from Uganda: 42 children aged under the age of 3 years and 40 children aged between 3 and 5 years. The infection status of these children had been previously determined by inspection of quadruplicate Kato-Katz faecal smears, a single urine circulating cathodic antigen (CCA) dipstick and antibody titres to S. mansoni soluble egg antigen (SmSEA) with a commercially available ELISA.
RESULTS
Upon comparison with quadruplicate Kato-Katz the sensitivity and specificity of the RDT were 75.7% and 31.1%, respectively. When using the SmSEA-ELISA as an alternate reference test, the RDT achieved 81.3% sensitivity and 61.1% specificity. Sensitivity and specificity compared to the urine-CCA test was 74.5% and 32.3% respectively. Sensitivity differed significantly according to age group.
CONCLUSIONS
The performance of the RDT within this study appeared favourable when compared with the currently-available SmSEA-ELISA. Looking to the future a serological POC test would be particularly promising for use in disease mapping in younger children especially in guiding administration of praziquantel treatment in selective treatment settings.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies QY Clinical Pathology > Diagnostic Tests > QY 250 Immunodiagnostic tests WC Communicable Diseases > Tropical and Parasitic Diseases > WC 698 Parasitic intestinal diseases (General) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 810 Schistosomiasis |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1093/trstmh/trt077 |
| Depositing User: | Mary Creegan |
| Date Deposited: | 11 Nov 2014 15:05 |
| Last Modified: | 02 Dec 2019 12:52 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/4539 |
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