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Poster Sessions: P0112 Effect of T follicular helper cells on regulation of mucosal immunity to influenza haemagglutinin by novel immunological adjuvants

Aljurayyan, A., Kasbekar, A., Beer, H., McCormick, M., Gordon, Stephen ORCID: and Zhang, Q. (2012) 'Poster Sessions: P0112 Effect of T follicular helper cells on regulation of mucosal immunity to influenza haemagglutinin by novel immunological adjuvants'. Immunology, Vol 137, Issue Supp 1, p. 222.

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Stimulation of the innate immune system is known to have an important role in the initiation and regulation of adaptive immunity. Therefore, inclusion of some immunological adjuvants such as Toll-like receptor ligands, which trigger early innate responses to enhance the adaptive responses, is crucial to vaccine effectiveness. T follicular helper cells (TFH) have recently been shown to be crucial in germinal centre function and in regulation of adaptive
immunity. The aim of the study is to investigate whether and how TLR ligands regulate T and B cell immunity to some respiratory tract pathogens through TFH cells.

Materials and methods:
Peripheral blood, nasopharyngeal swab and adenotonsillar tissues were collected form children and adults under going adenotonsillectomy. B cell antibody production analysed ELISA.TFH cells and effect of TLR ligands on their function were analysed by flowcytometry and intracellular cytokine staining. Kinetics of antibody and cytokine production will also be analysed by ELISA.

The proportion of TFH cells in adenotonsillar tissue was significantly higher in children than that in adults. Stimulation of adenotonsillar cells by CpG-DNA was shown to increase the numbers of TFH cells and this was consistent with the finding that CpG-DNA significantly enhance the antibody production to hae-moglutinin (HA) of seasonal influenza virus H1N1 (sH1N1) antigen in adenotonsillar cells. However, TLR-2 ligand (BLP) seems to downregulate both the proportion of TFH cells and the antibody level to sH1N1.

CpG-DNA promotes TFH cells in nasal-associated lymphoid tissue which is correlated with the enhancement of influenza HA-specific antibody production. Understanding the mechanisms by which TLR ligands regulate adaptive immunity through TFH cells may lead to novel vaccines against respiratory infections.

Item Type: Article
Additional Information: Special Issue: Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland
Subjects: QU Biochemistry > Cells and Genetics > QU 375 Cell physiology
QW Microbiology and Immunology > Reference Works. General Immunology > QW 520 Research (General)
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Digital Object Identifer (DOI):
Depositing User: Lynn Roberts-Maloney
Date Deposited: 10 Dec 2014 11:12
Last Modified: 07 Oct 2019 08:24


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