Detection of IgG antibodies in sera from patients with Cryptosporidium parvum and Cryptosporidium hominis

Chalmers, Rachel M., Elwin, Kristin, Cheesbrough, John, Hadfield, Stephen J. and Beeching, Nicholas ORCID: https://orcid.org/0000-0002-7019-8791 (2013) 'Detection of IgG antibodies in sera from patients with Cryptosporidium parvum and Cryptosporidium hominis'. Journal of Infection, Vol 67, Issue 3, pp. 231-237.

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Abstract

Objectives

Detection of anti-Cryptosporidium immunoglobulin G (IgG) antibodies in human sera has been used to demonstrate population exposure to this gastro-intestinal protozoan parasite. We characterised the dynamics of IgG antibody responses to two Cryptosporidium parvum (IOWA isolate) sporozoite antigens (15/17 kDa and 27 kDa) using longitudinal sera taken from laboratory-confirmed cryptosporidiosis cases in England and Wales. The effect of the infecting Cryptosporidium species was also investigated.

Methods

A mini-gel Western blot was used to test sera from ten Cryptosporidium stool-positive diarrhoea patients, taken soon after diagnosis and at 3 month intervals.

Results

Overall responses to the 15/17 kDa antigen complex were stronger and over a greater range than those to the 27 kDa antigen, but declined between 181 and 240 days and were barely detectable thereafter. Responses to the 27 kDa antigen were much weaker but remained detectable for a greater length of time. No differences were detected in either antibody response to infection with C. hominis or C. parvum.

Conclusions

The assay appears to be applicable for the study of recent exposure to C. parvum or C. hominis in the United Kingdom population, with strong responses to the 15/17 kDa antigen occurring within 6 months of infection.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 573 Antigens QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies QX Parasitology > Protozoa > QX 123 Apicomplexa QY Clinical Pathology > Diagnostic Tests > QY 250 Immunodiagnostic tests
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.jinf.2013.04.019
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	16 Feb 2015 15:55
Last Modified:	09 Sep 2019 06:27
URI:	https://archive.lstmed.ac.uk/id/eprint/4909

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