Mitchell, Sara N., Rigden, Daniel J., Dowd, Andrew, Lu, Fang, Wilding, Craig S., Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388, Dadzie, Samuel, Jenkins, Adam M., Regna, Kimberly, Boko, Pelagie, Djogbenou, Luc, Muskavitch, Marc A. T., Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247, Paine, Mark ORCID: https://orcid.org/0000-0003-2061-7713, Mayans, Olga and Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497 (2014) 'Metabolic and Target-Site Mechanisms Combine to Confer Strong DDT Resistance in Anopheles gambiae'. PLoS ONE, Vol 9, Issue 3, e92662.
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Abstract
The development of resistance to insecticides has become a classic exemplar of evolution occurring within human time scales. In this study we demonstrate how resistance to DDT in the major African malaria vector Anopheles gambiae is a result of both target-site resistance mechanisms that have introgressed between incipient species (the M- and S-molecular forms) and allelic variants in a DDT-detoxifying enzyme. Sequencing of the detoxification enzyme, Gste2, from DDT resistant and susceptible strains of An. gambiae, revealed a non-synonymous polymorphism (I114T), proximal to the DDT binding domain, which segregated with strain phenotype. Recombinant protein expression and DDT metabolism analysis revealed that the proteins from the susceptible strain lost activity at higher DDT concentrations, characteristic of substrate inhibition. The effect of I114T on GSTE2 protein structure was explored through X-ray crystallography. The amino acid exchange in the DDT-resistant strain introduced a hydroxyl group nearby the hydrophobic DDT-binding region. The exchange does not result in structural alterations but is predicted to facilitate local dynamics and enzyme activity. Expression of both wild-type and 114T alleles the allele in Drosophila conferred an increase in DDT tolerance. The 114T mutation was significantly associated with DDT resistance in wild caught M-form populations and acts in concert with target-site mutations in the voltage gated sodium channel (Vgsc-1575Y and Vgsc-1014F) to confer extreme levels of DDT resistance in wild caught An. gambiae.
Item Type: | Article |
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Subjects: | QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) |
Faculty: Department: | Biological Sciences > Vector Biology Department |
Digital Object Identifer (DOI): | https://doi.org/10.1371/journal.pone.0092662 |
Depositing User: | Lynn Roberts-Maloney |
Date Deposited: | 20 Apr 2015 10:33 |
Last Modified: | 16 Sep 2019 09:17 |
URI: | https://archive.lstmed.ac.uk/id/eprint/5093 |
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