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Kouassi, Bernard L., de Souza, Dziedzom K., Goepogui, Andre, Narh, Charles A., King, Sandra A., Mamadou, Baldé S., Diakité, Lamia, Dadzie, Samuel K., Boakye, Daniel A., Utzinger, Jürg, Bockarie, Moses and Koudou, Benjamin (2015) 'Assessing the presence of Wuchereria bancrofti in vector and human populations from urban communities in Conakry, Guinea'. Parasites & Vectors, Vol 8, e492.
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Abstract
Background
The Global Programme to Eliminate Lymphatic Filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (LF) through multiple rounds of mass drug administration (MDA). In Guinea, there is evidence of ongoing LF transmission, but little is known about the most densely populated parts of the country, including the capital Conakry. In order to guide the LF control and elimination efforts, serological and entomological surveys were carried out to determine whether or not LF transmission occurs in Conakry.
Methods
The prevalence of circulating filarial antigen (CFA) of Wuchereria bancrofti was assessed by an immuno-chromatography test (ICT) in people recruited from all five districts of Conakry. Mosquitoes were collected over a 1-year period, in 195 households in 15 communities. A proportion of mosquitoes were analysed for W. bancrofti, using dissection, loop-mediated isothermal amplification (LAMP) assay and conventional polymerase chain reaction (PCR).
Results
CFA test revealed no infection in the 611 individuals examined. A total of 14,334 mosquitoes were collected; 14,135 Culex (98.6 %), 161 Anopheles (1.1 %) and a few other species. Out of 1,312 Culex spp. (9.3 %) and 51 An. gambiae (31.7 %) dissected, none was infected with any stage of the W. bancrofti parasite. However, the LAMP assay revealed that 1.8 % of An. gambiae and 0.31 % of Culex spp. were positive, while PCR determined respective prevalences of 0 % and 0.19 %.
Conclusions
This study revealed the presence of W. bancrofti DNA in mosquitoes, despite the apparent absence of infection in the human population. Although MDA interventions are not recommended where the prevalence of ICT is below 1 %, the entomological results are suggestive of the circulation of the parasite in the population of Conakry. Therefore, rigorous surveillance is still warranted so that LF transmission in Conakry would be identified rapidly and adequate responses being implemented.
| Item Type: | Article |
|---|---|
| Additional Information: | The electronic version of this article is the complete one and can be found online at: http://www.parasitesandvectors.com/content/8/1/492 |
| Subjects: | QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General) |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1186/s13071-015-1077-x |
| Depositing User: | Lynn Roberts-Maloney |
| Date Deposited: | 03 Nov 2015 10:53 |
| Last Modified: | 17 Dec 2021 11:51 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/5393 |
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