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Kulohoma, Benard W, Cornick, Jennifer E, Chaguza, Chrispin, Yalcin, Feyruz, Harris, Simon R, Gray, Katherine J, Kiran, Anmol M, Molyneux, Elizabeth, French, Neil, Parkhill, Julian, Faragher, Brian, Everett, Dean B, Bentley, Stephen D and Heyderman, Robert S (2015) 'Comparative Genomic Analysis of Meningitis- and Bacteremia-Causing Pneumococci Identifies a Common Core Genome'. Infection and Immunity, Vol 83, Issue 10, pp. 4165-4173.
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Abstract
Streptococcus pneumoniae is a nasopharyngeal commensal that occasionally invades normally sterile sites to cause bloodstream infection and meningitis. Although the pneumococcal population structure and evolutionary genetics are well defined, it is not clear whether pneumococci that cause meningitis are genetically distinct from those that do not. Here, we used whole-genome sequencing of 140 isolates of S. pneumoniae recovered from bloodstream infection (n = 70) and meningitis (n = 70) to compare their genetic contents. By fitting a double-exponential decaying-function model, we show that these isolates share a core of 1,427 genes (95% confidence interval [CI], 1,425 to 1,435 genes) and that there is no difference in the core genome or accessory gene content from these disease manifestations. Gene presence/absence alone therefore does not explain the virulence behavior of pneumococci that reach the meninges. Our analysis, however, supports the requirement of a range of previously described virulence factors and vaccine candidates for both meningitis- and bacteremia-causing pneumococci. This high-resolution view suggests that, despite considerable competency for genetic exchange, all pneumococci are under considerable pressure to retain key components advantageous for colonization and transmission and that these components are essential for access to and survival in sterile sites.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Genetics > QU 460 Genomics. Proteomics WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 240 Bacteremia. Sepsis. Toxemias WL Nervous System > WL 200 Meninges. Blood-brain barrier |
| Faculty: Department: | Clinical Sciences & International Health > Clinical Sciences Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1128/IAI.00814-15 |
| Depositing User: | Jessica Jones |
| Date Deposited: | 07 Jan 2016 16:41 |
| Last Modified: | 06 Feb 2018 13:11 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/5480 |
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