Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles

Kunda, Nitesh K, Alfagih, Iman M, Miyaji, Eliane N, Figueiredo, Douglas B, Gonçalves, Viviane M, Ferreira, Daniela ORCID: https://orcid.org/0000-0002-0594-0902, Dennison, Sarah R, Somavarapu, Satyanarayana, Hutcheon, Gillian A and Saleem, Imran Y (2015) 'Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles'. International Journal of Pharmaceutics, Vol 495, Issue 2, pp. 903-912.

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Abstract

Pneumonia, caused by Streptococcus pneumoniae, mainly affects the immunocompromised, the very young and the old, and remains one of the leading causes of death. A steady rise in disease numbers from non-vaccine serotypes necessitates a new vaccine formulation that ideally has better antigen stability and integrity, does not require cold-chain and can be delivered non-invasively. In this study, a dry powder vaccine containing an important antigen of S. pneumoniae, pneumococcal surface protein A (PspA) that has shown cross-reactivity amongst serotypes to be delivered via the pulmonary route has been formulated. The formulation contains the antigen PspA adsorbed onto the surface of polymeric nanoparticles encapsulated in l-leucine microparticles that can be loaded into capsules and delivered via an inhaler. We have successfully synthesized particles of ∼150 nm and achieved ∼20 μg of PspA adsorption per mg of NPs. In addition, the spray-dried powders displayed a FPF of 74.31 ± 1.32% and MMAD of 1.70 ± 0.03 μm suggesting a broncho-alveolar lung deposition facilitating the uptake of the nanoparticles by dendritic cells. Also, the PspA released from the dry powders maintained antigen stability (SDS-PAGE), integrity (Circular dichroism) and activity (lactoferrin binding assay). Moreover, the released antigen also maintained its antigenicity as determined by ELISA.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 573 Antigens WB Practice of Medicine > Therapeutics > WB 340 Drug Administration WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 204 Pneumococcal pneumonia. Staphylococcal pneumonia WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.ijpharm.2015.09.034
Depositing User:	Jessica Jones
Date Deposited:	25 Jan 2016 12:47
Last Modified:	05 Nov 2019 14:56
URI:	https://archive.lstmed.ac.uk/id/eprint/5515

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