Scriven, James, Graham, Lisa, Schutz, Charlotte, Scriba, Thomas J, Wilkinson, Katalin A, Wilkinson, Robert J, Boulware, David R, Urban, Britta ORCID: https://orcid.org/0000-0002-4197-8393, Lalloo, David ORCID: https://orcid.org/0000-0001-7680-2200 and Meintjes, Graham (2016) 'A Glucuronoxylomannan-Associated Immune Signature, Characterized by Monocyte Deactivation and an Increased Interleukin 10 Level, Is a Predictor of Death in Cryptococcal Meningitis'. Journal of Infectious Disease, Vol 213, Issue 11, pp. 1725-1734.
Full text not available from this repository.Abstract
Background.
Cryptococcal meningitis remains a significant cause of death among human immunodeficiency virus type 1 (HIV)–infected persons in Africa. We aimed to better understand the pathogenesis and identify immune correlates of mortality, particularly the role of monocyte activation.
Methods.
A prospective cohort study was conducted in Cape Town, South Africa. Patients with a first episode of cryptococcal meningitis were enrolled, and their immune responses were assessed in unstimulated and stimulated blood specimens, using flow cytometry and cytokine analysis.
Results.
Sixty participants were enrolled (median CD4+ T-cell count, 34 cells/µL). Mortality was 23% (14 of 60 participants) at 14 days and 39% (22 of 57) at 12 weeks. Nonsurvivors were more likely to have an altered consciousness and higher cerebrospinal fluid fungal burden at presentation. Principal component analysis identified an immune signature associated with early mortality, characterized by monocyte deactivation (reduced HLA-DR expression and tumor necrosis factor α response to lipopolysaccharide); increased serum interleukin 6, CXCL10, and interleukin 10 levels; increased neutrophil counts; and decreased T-helper cell type 1 responses. This immune signature remained an independent predictor of early mortality after adjustment for consciousness level and fungal burden and was associated with higher serum titers of cryptococcal glucuronoxylomannan.
Conclusions.
Cryptococcal-related mortality is associated with monocyte deactivation and an antiinflammatory blood immune signature, possibly due to Cryptococcus modulation of the host immune response. Validation in other cohorts is required.
Item Type: | Article |
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Subjects: | QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance. WA Public Health > Statistics. Surveys > WA 900 Public health statistics WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503.5 Complications WL Nervous System > WL 200 Meninges. Blood-brain barrier |
Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology Clinical Sciences & International Health > Clinical Sciences Department Clinical Sciences & International Health > International Public Health Department |
Digital Object Identifer (DOI): | https://doi.org/10.1093/infdis/jiw007 |
Depositing User: | Jessica Jones |
Date Deposited: | 14 Mar 2016 09:54 |
Last Modified: | 07 Dec 2018 10:17 |
URI: | https://archive.lstmed.ac.uk/id/eprint/5756 |
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