Trypanosoma brucei AMP-activated kinase subunit homologs influence surface molecule expression

Clemmens, Clarice S, Morris, Meredith T, Lyda, Todd A, Acosta Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959 and Morris, James C (2009) 'Trypanosoma brucei AMP-activated kinase subunit homologs influence surface molecule expression'. Experimental Parasitology, Vol 123, Issue 3, pp. 250-7.

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Abstract

The African trypanosome, Trypanosoma brucei, can gauge its environment by sensing nutrient availability. For example, procyclic form (PF) trypanosomes monitor changes in glucose levels to regulate surface molecule expression, which is important for survival in the tsetse fly vector. The molecular connection between glycolysis and surface molecule expression is unknown. Here we partially characterize T. brucei homologs of the beta and gamma subunits of the AMP-activated protein kinase (AMPK), and determine their roles in regulating surface molecule expression. Using flow cytometry and mass spectrometry, we found that TbAMPKbeta or TbAMPKgamma-deficient parasites express both of the major surface molecules, EP- and GPEET-procyclin, with the latter being a form that is expressed when glucose is low such as in the tsetse fly. Last, we have found that the putative scaffold component of the complex, TbAMPKbeta, fractionates with organellar components and colocalizes in part with a glycosomal marker as well as the flagellum of PF parasites.

Item Type:	Article
Uncontrolled Keywords:	Trypanosoma brucei African tryopanosome nutrient sensing procyclins mass spectrometry
Subjects:	QX Parasitology > Insects. Other Parasites > QX 505 Diptera WC Communicable Diseases > Tropical and Parasitic Diseases > WC 680 Tropical diseases (General) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WC Communicable Diseases > Tropical and Parasitic Diseases > WC 695 Parasitic diseases (General)
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.exppara.2009.07.010
Depositing User:	Users 183 not found.
Date Deposited:	03 Jun 2010 15:44
Last Modified:	14 Dec 2021 10:29
URI:	https://archive.lstmed.ac.uk/id/eprint/676

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