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Charlier, J., De Meulemeester, L., Claerebout, E., Williams, Diana J.L. and Vercruysse, J. (2008) 'Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle'. Veterinary Parasitology, Vol 153, Issue 1-2, pp. 44-51.
Full text not available from this repository.Abstract
The objectives of this study were (1) to evaluate available coprological and serological tests for detection of Fasciola hepatica infection in field conditions, (2) to investigate if the season when samples were collected affects the interpretation of the test results, and (3) to evaluate if the test results are associated with the level of infection. During weekly visits to an abattoir, the whole liver, a rectal faecal sample and a blood sample were collected from 100 cows in two seasons each ("spring" = February-May 2006 and "autumn" = October-December 2006). A sedimentation-flotation technique on 4 g (SF 4 g) or 10 g (SF 10 g) of faeces, a copro-antigen ELISA and two indirect serum F hepatica ELISAs (excretory-secretory (ES) and Pourquier ELISA) were performed and the test results were compared with the presence of infection and worm counts at liver necropsy. Over both seasons the sensitivity (Se) and specificity (Sp) were for the SF 4 g 43% and 100%, for the SF 10 g 64% and 93%, for the copro-antigen ELISA 94% and 93%, for the ES ELISA 87% and 90% and for the Pourquier ELISA 88% and 84%. Significant between-season differences (P < 0.05) were observed in the sensitivities of the two serological ELISAs: whereas the Pourquier ELISA had a higher sensitivity in spring than in autumn, the opposite was true for the ES ELISA. There were no significant between-season differences in the specificity for any of the tests. The test results of the SF 4 g, copro-antigen ELISA and ES ELISA were associated with the level of infection of the animal. Given a positive test result of the SF 4 g it is at least I I times more likely that the animal is carrying a heavy infection (> 10 flukes) than that is free of infection or lightly infected (< 10 flukes). Weak (similar to 0.3) and moderate (similar to 0.6) correlations were observed within infected animals of level of infection with ES and copro-antigen ELISA results, respectively. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cattle trematoda fasciola hepatica sedimentation-flotation elisa copro-antigen ggt morbidity level of infection linked-immunosorbent-assay hepatica infection liver fluke elisa sheep tests herds |
| Subjects: | QX Parasitology > Helminths. Annelida > QX 353 Trematoda |
| Faculty: Department: | Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008) |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.vetpar.2008.01.035 |
| Depositing User: | Ms Julia Martin |
| Date Deposited: | 28 Jul 2010 09:30 |
| Last Modified: | 06 Feb 2018 13:00 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/750 |
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