A helicase-containing module defines a family of pCD630-like plasmids in Clostridium difficile

Smits, Wiep Klass, Weese, J. Scott, Roberts, Adam ORCID: https://orcid.org/0000-0002-0760-3088, Harmanus, Celine and Hornung, Bastien (2018) 'A helicase-containing module defines a family of pCD630-like plasmids in Clostridium difficile'. Anaerobe, Vol 49, pp. 78-84.

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Abstract

Clostridium difficile is a Gram-positive and sporulating enteropathogen that is a major cause of healthcare-associated infections. Even though a large number of genomes of this species have been sequenced, only a few plasmids have been described in the literature. Here, we use a combination of in silico analyses and laboratory experiments to show that plasmids are common in C. difficile. We focus on a group of plasmids that share similarity with the plasmid pCD630, from the reference strain 630. The family of pCD630-like plasmids is defined by the presence of a conserved putative helicase that is likely part of the plasmid replicon. This replicon is compatible with at least some other C. difficile replicons, as strains can carry pCD630-like plasmids in addition to other plasmids. We find two distinct sub-groups of pCD630-like plasmids that differ in size and accessory modules. This study is the first to describe a family of plasmids in C. difficile.

Item Type:	Article
Subjects:	QU Biochemistry > Enzymes > QU 137 Isomerases QU Biochemistry > Genetics > QU 470 Genetic structures QW Microbiology and Immunology > Bacteria > QW 142 Gram-positive bacteria (General) QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics. WC Communicable Diseases > Infection. Bacterial Infections > Other Bacterial Infections. Zoonotic Bacterial Infections > WC 368 Clostridium infections
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.anaerobe.2017.12.005
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	15 Dec 2017 09:39
Last Modified:	06 Sep 2019 09:34
URI:	https://archive.lstmed.ac.uk/id/eprint/7959

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