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Unwin, Vera, Ainsworth, Shaun, Rippon, Emily, Niang, El Hadji Amadou, Paine, Mark 
ORCID: https://orcid.org/0000-0003-2061-7713, Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388 and Adams, Emily ORCID: https://orcid.org/0000-0002-0816-2835
(2018)
'Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae.'. Parasites & Vectors, Vol 11, Issue 1, p. 307.
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Abstract
BACKGROUND
The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics.
METHODS
A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae. Developed on the portable, rapid, point-of-care compatible PCR platform - Genedrive® (genedrive® plc), the test comprises two assays which target single nucleotide polymorphisms (SNPs) in the voltage gated sodium channel (VGSC) gene that exert interactive effects on knockdown resistance (kdr): L1014F, L1014S and N1575Y.
RESULTS
Distinct melt peaks were observed for each allele at each locus. Preliminary validation of these assays using a test panel of 70 An. gambiae samples showed complete agreement of our assays with the widely-used TaqMan assays, achieving a sensitivity and specificity of 100%.
CONCLUSION
Here we show the development of an insecticide resistance detection assay for use on the Genedrive® platform that has the potential to be the first field-applicable diagnostic for kdr.
| Item Type: | Article |
|---|---|
| Subjects: | QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1186/s13071-018-2893-6 |
| Depositing User: | Stacy Murtagh |
| Date Deposited: | 29 May 2018 15:40 |
| Last Modified: | 07 Sep 2019 13:08 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/8704 |
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