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Muller, Pie, Warr, E., Stevenson, Bradley, Pignatelli, Patricia, Morgan, John, Steven, Andrew, Yawson, A. E., Mitchell, Sara N., Ranson, Hilary 
ORCID: https://orcid.org/0000-0003-2332-8247, Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173, Paine, Mark ORCID: https://orcid.org/0000-0003-2061-7713 and Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497
(2008)
'Field-Caught Permethrin-Resistant Anopheles gambiae Overexpress CYP6P3, a P450 That Metabolises Pyrethroids'. PLoS Genetics, Vol 4, Issue 11, e1000286.
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Abstract
Insects exposed to pesticides undergo strong natural selection and have developed various adaptive mechanisms to survive. Resistance to pyrethroid insecticides in the malaria vector Anopheles gambiae is receiving increasing attention because it threatens the sustainability of malaria vector control programs in sub-Saharan Africa. An understanding of the molecular mechanisms conferring pyrethroid resistance gives insight into the processes of evolution of adaptive traits and facilitates the development of simple monitoring tools and novel strategies to restore the efficacy of insecticides. For this purpose, it is essential to understand which mechanisms are important in wild mosquitoes. Here, our aim was to identify enzymes that may be important in metabolic resistance to pyrethroids by measuring gene expression for over 250 genes potentially involved in metabolic resistance in phenotyped individuals from a highly resistant, wild A. gambiae population from Ghana. A cytochrome P450, CYP6P3, was significantly overexpressed in the survivors, and we show that the translated enzyme metabolises both alpha-cyano and non-alpha-cyano pyrethroids. This is the first study to demonstrate the capacity of a P450 identified in wild A. gambiae to metabolise insecticides. The findings add to the understanding of the genetic basis of insecticide resistance in wild mosquito populations.
| Item Type: | Article |
|---|---|
| Subjects: | WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors QX Parasitology > Insects. Other Parasites > QX 515 Anopheles |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1371/journal.pgen.1000286 |
| Depositing User: | Users 183 not found. |
| Date Deposited: | 05 Jul 2010 09:48 |
| Last Modified: | 17 Jul 2020 10:58 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/875 |
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