Subramaniam, Krishanthi, Austin, Victoria, Schocker, Nathaniel S., Montoya, Alba L., Anderson, Matthew S., Ashmus, Roger A., Mesri, Mina, Al-Salem, Waleed, Almeida, Igor C., Michael, Katja and Acosta Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959 (2018) 'Anti-α-Gal antibodies detected by novel neoglycoproteins as a diagnostic tool for Old World cutaneous leishmaniasis caused by Leishmania major'. Parasitology, Vol 145, Issue 13, pp. 1758-1764.
Full text not available from this repository.Abstract
Outbreaks of Old World cutaneous leishmaniasis (CL) have significantly increased due to the conflicts in the Middle East, with most of the cases occurring in resource-limited areas such as refugee settlements. The standard methods of diagnosis include microscopy and parasite culture, which have several limitations. To address the growing need for a CL diagnostic that can be field applicable, we have identified five candidate neoglycoproteins (NGPs): Galα (NGP3B), Galα(1,3)Galα (NGP17B), Galα(1,3)Galβ (NGP9B), Galα(1,6)[Galα(1,2)]Galβ (NGP11B), and Galα(1,3)Galβ(1,4)Glcβ (NGP1B) that are differentially recognized in sera from individuals with Leishmania major infection as compared with sera from heterologous controls. These candidates contain terminal, non-reducing α-galactopyranosyl (α-Gal) residues, which are known potent immunogens to humans. Logistic regression models found that NGP3B retained the best diagnostic potential (area under the curve from receiver-operating characteristic curve = 0.8). Our data add to the growing body of work demonstrating the exploitability of the human anti-α-Gal response in CL diagnosis
Item Type: | Article |
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Additional Information: | Special Issue 13 (2017 Autumn Symposium of the British Society for Parasitology The multi-disciplinarity of parasitology: Host-parasite evolution in an ever changing world) |
Subjects: | WA Public Health > WA 20.5 Research (General) WA Public Health > Health Problems of Special Population Groups > WA 300 General. Refugees WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis |
Faculty: Department: | Biological Sciences > Vector Biology Department |
Digital Object Identifer (DOI): | https://doi.org/10.1017/s0031182018000860 |
SWORD Depositor: | JISC Pubrouter |
Depositing User: | Stacy Murtagh |
Date Deposited: | 19 Jun 2018 16:08 |
Last Modified: | 01 Jun 2021 14:25 |
URI: | https://archive.lstmed.ac.uk/id/eprint/8793 |
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