LSTM Home > LSTM Research > LSTM Online Archive

Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle

Salimi-Bejestani, M. R., Cripps, P. and Williams, Diana J.L. (2008) 'Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle'. Veterinary Record, Vol 162, Issue 4, pp. 109-111.

Full text not available from this repository.


An ELISA with a diagnostic sensitivity of 98 per cent and specificity of 96 per cent was evaluated as a means of assessing the intensity of Fasciola hepatica infection in cattle. A total of 294 blood samples were collected from infected cattle at a local abattoir, and the level of infection in each animal was assessed on the basis of the extent of liver pathology and the presence of flukes; 120 blood samples were also collected from uninfected cattle kept on a farm known to be free of F hepatica. The results indicated that there was a significant correlation (P<0.001) between the ELISA values and the intensity of infection. Values between 15 and 28 per cent of a positive control sample indicated a low intensity of infection, values between 28 and 50 per cent indicated a medium intensity of infection and values above 50 per cent indicated a high intensity of infection.

Item Type: Article
Uncontrolled Keywords: linked-immunosorbent-assay liver fluke antibody resistance calves sheep diagnosis antigens england wales
Subjects: QX Parasitology > QX 20 Research (General)
QX Parasitology > Helminths. Annelida > QX 365 Fasciola
Faculty: Department: Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008)
Depositing User: Ms Julia Martin
Date Deposited: 01 Jul 2010 15:21
Last Modified: 06 Feb 2018 13:00


View details

Actions (login required)

Edit Item Edit Item