Detection of Streptococcus pneumoniae from culture-negative dried blood spots by real-time PCR in Nigerian children with acute febrile illness.

Iroh Tam, Pui-Ying ORCID: https://orcid.org/0000-0002-3682-8892, Hernandez-Alvarado, Nelmary, Schleiss, Mark R, Yi, Amy J, Hassan-Hanga, Fatimah, Onuchukwu, Chuma, Umoru, Dominic and Obaro, Stephen K (2018) 'Detection of Streptococcus pneumoniae from culture-negative dried blood spots by real-time PCR in Nigerian children with acute febrile illness.'. BMC Research Notes, Vol 11, Issue 1, p. 657.

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Abstract

OBJECTIVES
Acute febrile illness is a common cause of hospital admission, and its associated infectious causes, of which a key bacterial causative agent is Streptococcus pneumoniae, contribute to substantial morbidity and mortality. We sought to evaluate the utility of real-time (rt)-PCR on dried blood spots (DBS) for diagnosis of S. pneumoniae in acute febrile illness among children presenting to hospitals in Nigeria. We previously described preliminary results in a sample of 537 patients. Here we present data from a larger collection of 1038 patients.

RESULTS
Using rt-PCR for Streptococcus pneumoniae on 1038 dried blood spots from children prospectively enrolled with acute febrile illness, including 79 healthy controls, we detected pneumococcal DNA in nine of 15 blood culture-positive specimens, one culture-negative specimen from a high-risk group, a culture-confirmed non-pneumococcal specimen and a healthy control. Six culture-positive isolates (40%) were negative. Sensitivity was 60%, specificity 99.7%, positive predictive value 75% and negative predictive value 99.4%. Rt-PCR of DBS has limited sensitivity in blood specimens from acute febrile illness in children.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Bacteria > QW 142 Gram-positive bacteria (General) WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 202 Pneumonia (General or not elsewhere classified) WL Nervous System > WL 300 General works (Include works on brain alone) WS Pediatrics > WS 20 Research (General)
Faculty: Department:	Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW)
Digital Object Identifer (DOI):	https://doi.org/10.1186/s13104-018-3770-2
Depositing User:	Stacy Murtagh
Date Deposited:	18 Sep 2018 11:03
Last Modified:	18 Sep 2018 11:03
URI:	https://archive.lstmed.ac.uk/id/eprint/9343

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