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Snake Venom Gland Organoids

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Post, Y, Puschhof, J, Beumer, J, Kerkkamp, HM, de Bakker, MAG, Slagboom, J, de Barbanson, B, Wevers, NR, Spijkers, XM, Olivier, T, Kazandijan, Taline, Ainsworth, Stuart ORCID: https://orcid.org/0000-0002-0199-6482, Iglesias, CL, van de Wetering, WJ, Heinz, MC, van Ineveld, RL, van Kleef, RDGM, Begthel, H, Korving, J, Bar-Ephraim, YW, Getreuer, W, Rios, AC, Westerink, RHS, Snippert, HJG, van Oudenaarden, A, Peters, PJ, Vonk, FJ, Kool, J, Richardson, MK, Casewell, Nicholas ORCID: https://orcid.org/0000-0002-8035-4719 and Clevers, H (2020) 'Snake Venom Gland Organoids'. Cell, Vol 80, Issue 2, pp. 233-247.

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Abstract

Wnt dependency and Lgr5 expression define multiple mammalian epithelial stem cell types. Under defined growth factor conditions, such Adult Stem Cells (ASCs) grow as 3D organoids that recapitulate essential features of the pertinent epithelium. Here, we establish long-term expanding venom gland organoids from several snake species. The newly assembled transcriptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts. Single cell RNA sequencing of both organoids and primary tissue identifies distinct venom-expressing cell types as well as proliferative cells expressing homologs of known mammalian stem cell markers. A hard-wired regional heterogeneity in the expression of individual venom components is maintained in organoid cultures. Harvested venom peptides reflect crude venom composition and display biological activity. This study extends organoid technology to reptilian tissues and describes an experimentally tractable model system representing the snake venom gland.

Item Type: Article
Subjects: QU Biochemistry > Cells and Genetics > QU 300 General works
QU Biochemistry > Genetics > QU 475 Genetic processes
QU Biochemistry > Genetics > QU 550 Genetic techniques. PCR. Chromosome mapping
WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1016/j.cell.2019.11.038
Depositing User: Mary Creegan
Date Deposited: 24 Jan 2020 15:17
Last Modified: 24 Jan 2020 15:17
URI: https://archive.lstmed.ac.uk/id/eprint/13473

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