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A unicellular relative of animals generates a layer of polarized cells by actomyosin-dependent cellularization

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Dudin, Omaya, Ondracka, Andrej, Grau-Bové, Xavier, Haraldsen, Arthur AB, Toyoda, Atsushi, Suga, Hiroshi, Bråte, Jon and Ruiz-Trillo, Iñaki (2019) 'A unicellular relative of animals generates a layer of polarized cells by actomyosin-dependent cellularization'. eLife, Vol 8, e49801.

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Abstract

In animals, cellularization of a coenocyte is a specialized form of cytokinesis that results in the formation of a polarized epithelium during early embryonic development. It is characterized by coordinated assembly of an actomyosin network, which drives inward membrane invaginations. However, whether coordinated cellularization driven by membrane invagination exists outside animals is not known. To that end, we investigate cellularization in the ichthyosporean Sphaeroforma arctica, a close unicellular relative of animals. We show that the process of cellularization involves coordinated inward plasma membrane invaginations dependent on an actomyosin network and reveal the temporal order of its assembly. This leads to the formation of a polarized layer of cells resembling an epithelium. We show that this stage is associated with tightly regulated transcriptional activation of genes involved in cell adhesion. Hereby we demonstrate the presence of a self-organized, clonally-generated, polarized layer of cells in a unicellular relative of animals.

Item Type: Article
Subjects: QU Biochemistry > Cells and Genetics > QU 300 General works
QW Microbiology and Immunology > Immunity by Type > QW 568 Cellular immunity. Immunologic cytotoxicity. Immunocompetence. Immunologic factors (General)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.7554/eLife.49801
Depositing User: Stacy Murtagh
Date Deposited: 29 Oct 2019 15:36
Last Modified: 31 Oct 2019 11:13
URI: https://archive.lstmed.ac.uk/id/eprint/12928

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