Grigoraki, Linta ORCID: https://orcid.org/0000-0001-8997-0406, Grau-Bové, Xavier, Carrington Yates, Henrietta, Lycett, Gareth ORCID: https://orcid.org/0000-0002-2422-053X and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 (2020) 'Isolation and transcriptomic analysis of Anopheles gambiae oenocytes enables the delineation of hydrocarbon biosynthesis'. elife Science Publications Ltd, Vol 9, e58019.
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Grigoraki et al.,2020_eLife_accepted.docx - Accepted Version Available under License Creative Commons Attribution. Download (2MB) |
Abstract
The surface of insects is coated in cuticular hydrocarbons (CHCs); variations in the composition of this layer affect a range of traits including adaptation to arid environments and defence against pathogens and toxins. In the African malaria vector, Anopheles gambiae quantitative and qualitative variance in CHC composition have been associated with speciation, ecological habitat and insecticide resistance. Understanding how these modifications arise will inform us of how mosquitoes are responding to climate change and vector control interventions. CHCs are synthesised in sub-epidermal cells called oenocytes that are very difficult to isolate from surrounding tissues. Here we utilise a transgenic line with fluorescent oenocytes to purify these cells for the first time. Comparative transcriptomics revealed the enrichment of biological processes related to long chain fatty acyl-CoA biosynthesis and elongation of mono-, poly-unsaturated and saturated fatty acids and enabled us to delineate, and partially validate, the hydrocarbon biosynthetic pathway in An. gambiae.
Item Type: | Article |
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Subjects: | QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.7 RNA QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles |
Faculty: Department: | Biological Sciences > Vector Biology Department |
Digital Object Identifer (DOI): | https://doi.org/10.7554/eLife.58019 |
Depositing User: | Samantha Sheldrake |
Date Deposited: | 22 Jun 2020 10:39 |
Last Modified: | 13 Jul 2020 08:36 |
URI: | https://archive.lstmed.ac.uk/id/eprint/14765 |
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