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MondragonShem, Karina, Wongtrakul-Kish, Katherine, Kozak, Radoslaw P., Yan, Shi, Wilson, Iain B. H., Paschinger, Katharina, Rogers, Matthew E., Spencer, Daniel I. R. and Acosta-Serrano, Alvaro 
ORCID: https://orcid.org/0000-0002-2576-7959
(2020)
'Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis'. Scientific Reports, Vol 10, Issue 12903.
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Alvaro s41598-020-69753-x.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
During Leishmania transmission sand flies inoculate parasites and saliva into the skin of vertebrates. Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host’s immune responses. Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked. Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas. In silico predictions suggest half of Lu. longipalpis salivary proteins may be N-glycosylated. SDS-PAGE coupled to LC–MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins. To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC–MS/MS, MALDI-TOF–MS, and exoglycosidase treatments. We found that the N-glycan composition of Lu. longipalpis saliva mostly consists of oligomannose sugars, with Man5GlcNAc2 being the most abundant, and a few hybrid-type species. Interestingly, some glycans appear modified with a group of 144 Da, whose identity has yet to be confirmed. Our work presents the first detailed structural analysis of sand fly salivary glycans.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > QU 4 General works QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis |
| Faculty: Department: | Biological Sciences > Vector Biology Department Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1038/s41598-020-69753-x |
| Depositing User: | Cathy Waldron |
| Date Deposited: | 17 Sep 2020 13:59 |
| Last Modified: | 17 Sep 2020 13:59 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/15524 |
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