TZM-gfp cells: a tractable fluorescent tool for analysis of rare and early HIV-1 infection

Gludish, David W., Boliar, Saikat, Caldwell, Shannon, Tembo, Dumizulu L., Chimbayo, Elizabeth T., Jambo, Kondwani ORCID: https://orcid.org/0000-0002-3195-2210, Mwandumba, Henry ORCID: https://orcid.org/0000-0003-4470-3608 and Russell, David G. (2020) 'TZM-gfp cells: a tractable fluorescent tool for analysis of rare and early HIV-1 infection'. Scientific Reports, Vol 10, Issue 19900.

Preview

Text S_Reports published.pdf - Published Version Available under License Creative Commons Attribution. Download (5MB) | Preview

Abstract

Here we describe TZM-gfp, a novel HIV-1 reporter cell derived from the same parental clone JC.53, used previously to generate the widely-utilized indicator cell line TZM-bl. We re-engineered JC.53 cells to express GFP under regulation of HIV Tat and Rev. We characterize the new reporter cell line to show that TZM-gfp cells are equally susceptible to HIV infection, exhibit minimal background signal, and can report HIV infection in rare cells from a bulk population of experimentally-infected human monocyte-derived macrophages. We demonstrate the utility and sensitivity of the cells in detection of even a single HIV-positive macrophage by fluorescence-assisted correlative electron microscopy, using the GFP signal to guide imaging of HIV virions in primary co-culture. Finally, we used TZM-gfp cells for viral capture during co-culture with human peripheral blood mononuclear cells, showing that TZM-gfp can support outgrowth and analyses of patient-derived primary HIV-1 isolates.

Item Type:	Article
Subjects:	QU Biochemistry > Cells and Genetics > QU 300 General works WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503 Acquired immunodeficiency syndrome. HIV infections WH Hemic and Lymphatic Systems > Lymphatic System > WH 650 Reticuloendothelial system
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW)
Digital Object Identifer (DOI):	https://doi.org/10.1038/s41598-020-76422-6
Depositing User:	Marie Hatton
Date Deposited:	02 Dec 2020 13:45
Last Modified:	02 Dec 2020 13:45
URI:	https://archive.lstmed.ac.uk/id/eprint/16248

Statistics

View details

Actions (login required)

Edit Item