A novel, magnetic bead‐based extraction method for the isolation of antimicrobial resistance genes with a case study in river water in Malawi

Byrne, Rachel ORCID: https://orcid.org/0000-0001-5398-3125, Cocker, Derek, Aljayyoussi, Ghaith, Mphasa, Madalitso, Charles, Mary, Mandula, Tamandani, Williams, Chris, Rigby, Jonathan, Hearn, Jack ORCID: https://orcid.org/0000-0003-3358-4949, Feasey, Nicholas ORCID: https://orcid.org/0000-0003-4041-1405, Adams, Emily ORCID: https://orcid.org/0000-0002-0816-2835 and Edwards, Thomas (2022) 'A novel, magnetic bead‐based extraction method for the isolation of antimicrobial resistance genes with a case study in river water in Malawi'. Journal of Applied Microbiology, Vol 133, Issue 5, pp. 3191-3200.

Preview

Text jam.15755.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Aims: The environment is increasingly recognized as an important reservoir of antimicrobial resistance genes (ARGs), which can be identified using molecular platforms. Yet, environmental surveillance remains an underutilised tool as there is no agreement on the best strategy for sample processing. We aim to develop a low‐cost extraction method independent to commercial kits or reagents.
Methods and Results: We present a novel, magnetic bead‐based method for the isolation of ARGs from river water named MagnaExtract. We present this with analytic limit of detection as well as a case study in Southern Malawi. Here we compare the DNA yield from MagnaExtract with commercially available QIAGEN kits and the crude boil and spin method, using a high‐resolution melt analysis PCR panel designed for the detection of third‐generation cephalosporin and carbapenem‐resistant genes from 98 water samples.
Conclusion: The MagnaExtract method is comparable, and in some instance's superior to commercially available kits for the isolation of ARGs from river water samples. Significance and Impact of the Study: The MagnaExtract approach offers a simple, affordable, high yielding extraction method that could be used for the detection of ARGs from river water samples in surveillance campaigns in East Africa.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > QW 4 General works. Classify here works on microbiology as a whole. QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified. QW Microbiology and Immunology > Environmental Microbiology > QW 55 Environmental microbiology
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1111/jam.15755
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	18 Oct 2022 13:30
Last Modified:	06 Jun 2023 14:26
URI:	https://archive.lstmed.ac.uk/id/eprint/21034

Statistics

View details

Actions (login required)

Edit Item