Intracellular Transposition of Mobile Genetic Elements Associated with the Colistin Resistance Gene mcr-1

Goodman, Richard, Tansirichaiya, Supathep, Brouwer, Michael S M and Roberts, Adam ORCID: https://orcid.org/0000-0002-0760-3088 (2023) 'Intracellular Transposition of Mobile Genetic Elements Associated with the Colistin Resistance Gene mcr-1'. Microbiology Spectrum, Vol 11, Issue 1, e03278-22.

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Abstract

Mobile colistin resistance (mcr) genes are often located on conjugative plasmids, where their association with insertion sequences enables intercellular and intracellular dissemination throughout bacterial replicons and populations. Multiple mcr genes have been discovered in every habitable continent, in many bacterial species, on both plasmids and integrated into the chromosome. Previously, we showed the intercellular transfer of mcr-1 on an IncI1 plasmid, pMCR-E2899, between strains of Escherichia coli. Characterizing the intracellular dynamics of mcr-1 transposition and recombination would further our understanding of how these important genes move through bacterial populations and whether interventions can be put in place to stop their spread. In this study, we aimed to characterize transfer events from the mcr-1-containing transposon Tn7511 (ISApl1-mcr-1-pap2-ISApl1), located on plasmid pMCR-E2899, using the pBACpAK entrapment vector. Following the transformation of pBACpAK into our DH5α-Azir/pMCR-E2899 transconjugant, we captured ISApl1 in pBACpAK multiple times and, for the first time, observed the ISApl1-mediated transfer of the mcr-1 transposon (Tn7511) into the chromosome of E. coli DH5α. Whole-genome sequencing allowed us to determine consensus insertion sites of ISApl1 and Tn7511 in this strain, and comparison of these sites allowed us to explain the transposition events observed. These observations reveal the consequences of ISApl1 transposition within and between multiple replicons of the same cell and show mcr-1 transposition within the cell as part of the novel transposon Tn7511.

Item Type:	Article
Subjects:	QU Biochemistry > Cells and Genetics > QU 300 General works QU Biochemistry > Genetics > QU 470 Genetic structures QU Biochemistry > Genetics > QU 475 Genetic processes QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1128/spectrum.03278-22
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	17 Jan 2023 14:00
Last Modified:	03 Oct 2023 13:04
URI:	https://archive.lstmed.ac.uk/id/eprint/21694

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