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A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection

Donnellan, Samantha, Pennington, Shaun ORCID: https://orcid.org/0000-0002-7160-6275, Ruggiero, Alessandra, Martinez-Rodriguez, Carmen, Pouget, Marion, Thomas, Jordan, Ward, Steve ORCID: https://orcid.org/0000-0003-2331-3192, Pollakis, Georgios, Biagini, Giancarlo ORCID: https://orcid.org/0000-0001-6356-6595 and Paxton, William (2022) 'A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection'. Journal of Infectious Disease, Vol 227, Issue 5, pp. 708-713.

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Abstract

M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.

Item Type: Article
Subjects: WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503 Acquired immunodeficiency syndrome. HIV infections
WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503.5 Complications
WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1093/infdis/jiac491
Depositing User: Marie Hatton
Date Deposited: 12 Jan 2023 14:50
Last Modified: 04 Apr 2023 14:26
URI: https://archive.lstmed.ac.uk/id/eprint/21746

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